r/labrats • u/Elayouuu • 1d ago

Alternative for Sodium Thioglycolate ??

I have to make transport media for skin swabs, but my lab don't have sodium thioglycolate? Can you tell any alternative for this or do you know any other transport media I can make without it, Ames and Staurt both used sodium thioglycolate.

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u/kilobaser Microbiologist 1d ago

L-cysteine HCl could work. Maybe at 0.5mg/mL? I’m just trying to think of how much you’d need to work as reducing agent.

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u/Elayouuu 1d ago

I'm thinking of making ames medium in what we use 1 g per litre of sodium thioglycolate. How much L cysteine should I use and if you can, can you please share the procedure to make?

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u/idkthoughso 1d ago

Why did I instantly recall the legally blonde scene from just the name of the chemical?